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The Effect of Elevated Protein Intake on DNA Damage in Older People: Comparative Secondary Analysis of Two Randomized Controlled Trials

Authors :
Agnes Draxler
Bernhard Franzke
Johannes T. Cortolezis
Nicola A. Gillies
Sandra Unterberger
Rudolf Aschauer
Patrick A. Zöhrer
Laura Bragagna
Julia Kodnar
Eva-Maria Strasser
Oliver Neubauer
Pankaja Sharma
Sarah M. Mitchell
Nina Zeng
Farha Ramzan
Randall F. D’Souza
Scott O. Knowles
Nicole C. Roy
Anders M. Sjödin
Cameron J. Mitchell
Amber M. Milan
Barbara Wessner
David Cameron-Smith
Karl-Heinz Wagner
Source :
Nutrients, Vol 13, Iss 10, p 3479 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

A high protein intake at old age is important for muscle protein synthesis, however, this could also trigger protein oxidation with the potential risk for DNA damage. The aim of this study was to investigate whether an increased protein intake at recommended level or well above would affect DNA damage or change levels of reduced (GSH) and oxidised glutathione (GSSG) in community-dwelling elderly subjects. These analyses were performed in two randomized intervention studies, in Austria and in New Zealand. In both randomized control trials, the mean protein intake was increased with whole foods, in the New Zealand study (n = 29 males, 74.2 ± 3.6 years) to 1.7 g/kg body weight/d (10 weeks intervention; p < 0.001)) in the Austrian study (n = 119 males and females, 72.9 ± 4.8 years) to 1.54 g/kg body weight/d (6 weeks intervention; p < 0.001)). In both studies, single and double strand breaks and as formamidopyrimidine—DNA glycosylase-sensitive sites were investigated in peripheral blood mononuclear cells or whole blood. Further, resistance to H2O2 induced DNA damage, GSH, GSSG and CRP were measured. Increased dietary protein intake did not impact on DNA damage markers and GSH/GSSG levels. A seasonal-based time effect (p < 0.05), which led to a decrease in DNA damage and GSH was observed in the Austrian study. Therefore, increasing the protein intake to more than 20% of the total energy intake in community-dwelling seniors in Austria and New Zealand did not increase measures of DNA damage, change glutathione status or elevate plasma CRP.

Details

Language :
English
ISSN :
20726643
Volume :
13
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Nutrients
Publication Type :
Academic Journal
Accession number :
edsdoj.fc30b124036f4e4d8ed1c6890db9f787
Document Type :
article
Full Text :
https://doi.org/10.3390/nu13103479