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C-terminal domain small phosphatase-like 2 promotes epithelial-to-mesenchymal transition via Snail dephosphorylation and stabilization
- Source :
- Open Biology, Vol 8, Iss 4 (2018)
- Publication Year :
- 2018
- Publisher :
- The Royal Society, 2018.
-
Abstract
- The epithelial-to-mesenchymal transition (EMT) is a cellular reprogramming process converting epithelial cells into mesenchymal cell morphology. Snail is a critical regulator of EMT by both suppressing epithelial gene expression and promoting mesenchymal gene expression. Expression and activity of Snail are tightly controlled at transcriptional and post-translational levels. It has previously been reported that Snail undergoes phosphorylation and ubiquitin-dependent proteasome degradation. Here, we report nuclear phosphatase SCP4/CTDSPL2 acts as a novel Snail phosphatase. SCP4 physically interacts with and directly dephosphorylates Snail. SCP4-mediated dephosphorylation of Snail suppresses the ubiquitin-dependent proteasome degradation of Snail and consequently enhances TGFβ-induced EMT. The knockdown of SCP4 in MCF10A mammary epithelial cells leads to attenuated cell migration. Collectively, our finding demonstrates that SCP4 plays a critical role in EMT through Snail dephosphorylation and stabilization.
Details
- Language :
- English
- ISSN :
- 20462441
- Volume :
- 8
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- Open Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.fbcd26a8fab4f5e8f413463e117c3b3
- Document Type :
- article
- Full Text :
- https://doi.org/10.1098/rsob.170274