Back to Search Start Over

Diagnosis and characterization of the ribosomal DNA-ITS of potato rot nematode (Ditylenchus destructor) populations from Chinese medicinal herbs

Authors :
Chun-hui NI
Bian HAN
Yong-gang LIU
Maria MUNAWAR
Shi-ming LIU
Wen-hao LI
Ming-ming SHI
Hui-xia LI
De-liang PENG
Source :
Journal of Integrative Agriculture, Vol 22, Iss 6, Pp 1763-1781 (2023)
Publication Year :
2023
Publisher :
Elsevier, 2023.

Abstract

The potato rot nematode (Ditylenchus destructor) is a very economically important nematode in agronomic and horticultural plants worldwide. In this study, 43 populations of D. destructor were collected from different hosts across China, including 37 populations from Chinese herbal medicine plants. Obtained sequences of ITS-rDNA and D2–D3 of 28S-rDNA genes of D. destructor were compared and analyzed. Nine types of significant length variations in ITS sequences were observed among all populations. The differences in ITS1 length were mainly caused by the presence of repetitive elements with substantial base substitutions. Reconstructions of ITS1 secondary structures showed that the minisatellites formed a stem structure. Ten haplotypes were observed in all populations based on mutations and variations of helix H9. Among them, 3 known haplotypes (A–C) were found in 7 populations isolated from potato, sweet potato, and Codonopsis pilosula, and 7 unique haplotypes were found in other 36 populations collected from C. pilosula and Angelica sinensis compared with 7 haplotypes (A–G) according to Subbotin’ system. These unique haplotypes were different from haplotypes A–G, and we named them as haplotypes H–N. The present results showed that a total of 14 haplotypes (A–N) of ITS-rDNA have been found in D. destructor. Phylogenetic analyses of ITS-rDNA and D2–D3 showed that all populations of D. destructor were clustered into two major clades: one clade only containing haplotype A from sweet potato and the other containing haplotypes B–N from other plants. For further verification, PCR-ITS-RFLP profiles were conducted on 7 new haplotypes. Collectively, our study suggests that D. destructor populations on Chinese medicinal materials are very different from those on other hosts and this work provides a paradigm for relevant researches.

Details

Language :
English
ISSN :
20953119
Volume :
22
Issue :
6
Database :
Directory of Open Access Journals
Journal :
Journal of Integrative Agriculture
Publication Type :
Academic Journal
Accession number :
edsdoj.fa47399f0e3b462aa741152aa0d6bb26
Document Type :
article
Full Text :
https://doi.org/10.1016/j.jia.2022.08.126