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Calibration-free NGS quantitation of mutations below 0.01% VAF

Authors :
Peng Dai
Lucia Ruojia Wu
Sherry Xi Chen
Michael Xiangjiang Wang
Lauren Yuxuan Cheng
Jinny Xuemeng Zhang
Pengying Hao
Weijie Yao
Jabra Zarka
Ghayas C. Issa
Lawrence Kwong
David Yu Zhang
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

Quantification of rare somatic mutations is essential for basic research and translational applications. Here the authors present Quantitative Blocker Displacement Amplification allowing for accurate detection of mutations below 0.01% VAF.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.fa41048a06034246b3822d3ae3d81bf0
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-26308-6