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Dexmedetomidine Leads to the Mitigation of Myocardial Ischemia/Reperfusion-Induced Acute Lung Injury in Diabetic Rats Via Modulation of Hypoxia-Inducible Factor-1α
- Source :
- Brazilian Journal of Cardiovascular Surgery, Vol 37, Iss 3, Pp 370-379 (2022)
- Publication Year :
- 2022
- Publisher :
- Sociedade Brasileira de Cirurgia Cardiovascular, 2022.
-
Abstract
- ABSTRACT Introduction: The objective of this study is to investigate the protective mechanism of dexmedetomidine (Dex) in myocardial ischemia/reperfusion (MIR)-induced acute lung injury (ALI) of diabetic rats by inhibiting hypoxia-inducible factor-1α (HIF-1α). Methods: Initially, healthy male Sprague Dawley rats were treated with streptozocin to induce diabetes. Then, three weeks after the induction, Dex or lentiviral vector (LV)-HIF-1α was injected into the rats 30 minutes prior to the MIR modeling. After four weeks, lung tissues were harvested for pathological changes observation and the wet/dry weight (W/D) ratio determination. Afterwards, oxidative stress indicators and pro-inflammatory factors were measured. In addition, HIF-1α expression was assessed by immunohistochemistry and western blot analysis. Results: Dex could suppress inflammatory cell infiltration, improve lung tissue structure, reduce pathological score and the W/D ratio, and block oxidative stress and inflammatory response in MIR-induced ALI of diabetic rats. Besides, Dex could also inhibit HIF-1α expression. Moreover, Dex + LV-HIF-1α reversed the protective role of Dex on diabetic MIR-induced ALI. Conclusion: Our study has made it clear that Dex inhibited the upregulation of HIF-1α in diabetic MIR-induced ALI, and thus protect lung functions by quenching the accumulation of oxygen radical and reducing lung inflammatory response.
Details
- Language :
- English
- ISSN :
- 16789741
- Volume :
- 37
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Brazilian Journal of Cardiovascular Surgery
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.fa0e9202964c2d8e9628961f1edcb9
- Document Type :
- article
- Full Text :
- https://doi.org/10.21470/1678-9741-2020-0591