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Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

Authors :
Lisanne de Vor
Bruce van Dijk
Kok van Kessel
Jeffrey S Kavanaugh
Carla de Haas
Piet C Aerts
Marco C Viveen
Edwin C Boel
Ad C Fluit
Jakub M Kwiecinski
Gerard C Krijger
Ruud M Ramakers
Freek J Beekman
Ekaterina Dadachova
Marnix GEH Lam
H Charles Vogely
Bart CH van der Wal
Jos AG van Strijp
Alexander R Horswill
Harrie Weinans
Suzan HM Rooijakkers
Source :
eLife, Vol 11 (2022)
Publication Year :
2022
Publisher :
eLife Sciences Publications Ltd, 2022.

Abstract

Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and potential treatment of biofilm-related infections. Here, we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. The mAbs were also shown to bind a collection of clinical isolates derived from different biofilm-associated infections (endocarditis, prosthetic joint, catheter). We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. Furthermore, we show that a mAb recognizing wall teichoic acid (clone 4497) specifically localizes to a subcutaneously implanted pre-colonized catheter in mice. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo.

Details

Language :
English
ISSN :
2050084X
Volume :
11
Database :
Directory of Open Access Journals
Journal :
eLife
Publication Type :
Academic Journal
Accession number :
edsdoj.f9dfe179409a4ffabf19a218bc324d32
Document Type :
article
Full Text :
https://doi.org/10.7554/eLife.67301