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Dopamine Suppresses Osteogenic Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells via AKT/GSK-3β/β-Catenin Signaling Pathway

Authors :
Zhili Kuang
Zheng Chen
Shaoqin Tu
Zhihui Mai
Lin Chen
Xiaoning Kang
Xiaochuan Chen
Jiaming Wei
Yuxuan Wang
Yun Peng
Hong Ai
Source :
Stem Cells International, Vol 2022 (2022)
Publication Year :
2022
Publisher :
Hindawi Limited, 2022.

Abstract

Nervous system is critically involved in bone homeostasis and osteogenesis. Dopamine, a pivotal neurotransmitter, plays a crucial role in sympathetic regulation, hormone secretion, immune activation, and blood pressure regulation. However, the role of dopamine on osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) remains poorly understood. In this study, we firstly investigated the effect of dopamine on the apoptosis, proliferation, and osteogenic differentiation of rBMSCs. Dopamine did not, however, interfere with the apoptosis and proliferation of rBMSCs. Interestingly, dopamine suppressed the osteogenic differentiation of rBMSCs, as characterized by reduced ALP staining, ALP activity, mineralized nodule formation, and the mRNA and protein levels of osteogenesis-related genes (Col1a1, Alp, Runx2, Opn, and Ocn). Furthermore, dopamine inactivated AKT/GSK-3β/β-catenin signaling pathway. Treatment of LiCl (GSK-3β inhibitor) rescued the inhibitory effects of dopamine on osteogenic differentiation of rBMSCs. LY294002 (AKT inhibitor) administration exacerbated the inhibitory effects of dopamine on osteogenic differentiation of rBMSCs. Taken together, these findings indicate that dopamine suppresses osteogenic differentiation of rBMSCs via AKT/GSK-3β/β-catenin signaling pathway. Our study provides new insights into the role of neurotransmitters in bone homeostasis.

Subjects

Subjects :
Internal medicine
RC31-1245

Details

Language :
English
ISSN :
16879678
Volume :
2022
Database :
Directory of Open Access Journals
Journal :
Stem Cells International
Publication Type :
Academic Journal
Accession number :
edsdoj.f9c2c3022ee94476ba3e08f783cd19dc
Document Type :
article
Full Text :
https://doi.org/10.1155/2022/4154440