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Selective release of circRNAs in platelet-derived extracellular vesicles

Authors :
Christian Preußer
Lee-Hsueh Hung
Tim Schneider
Silke Schreiner
Martin Hardt
Anna Moebus
Sentot Santoso
Albrecht Bindereif
Source :
Journal of Extracellular Vesicles, Vol 7, Iss 1 (2018)
Publication Year :
2018
Publisher :
Wiley, 2018.

Abstract

Circular RNAs (circRNAs) are a novel class of noncoding RNAs present in all eukaryotic cells investigated so far and generated by a special mode of alternative splicing of pre-mRNAs. Thereby, single exons, or multiple adjacent and spliced exons, are released in a circular form. CircRNAs are cell-type specifically expressed, are unusually stable, and can be found in various body fluids such as blood and saliva. Here we analysed circRNAs and the corresponding linear splice isoforms from human platelets, where circRNAs are particularly abundant, compared with other hematopoietic cell types. In addition, we isolated extracellular vesicles from purified and in vitro activated human platelets, using density-gradient centrifugation, followed by RNA-seq analysis for circRNA detection. We could demonstrate that circRNAs are packaged and released within both types of vesicles (microvesicles and exosomes) derived from platelets. Interestingly, we observed a selective release of circRNAs into the vesicles, suggesting a specific sorting mechanism. In sum, circRNAs represent yet another class of extracellular RNAs that circulate in the body and may be involved in signalling pathways. Since platelets are essential for central physiological processes such as haemostasis, wound healing, inflammation and cancer metastasis, these findings should greatly extend the potential of circRNAs as prognostic and diagnostic biomarkers.

Details

Language :
English
ISSN :
20013078
Volume :
7
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Journal of Extracellular Vesicles
Publication Type :
Academic Journal
Accession number :
edsdoj.f9102faa45c94a298eed71fe8c9b5225
Document Type :
article
Full Text :
https://doi.org/10.1080/20013078.2018.1424473