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Antimicrobial Activity of Diverse Chemotypes of Lippia graveolens Against Aeromonas hydrophila Isolated from Oreochromis niloticus
- Source :
- Uniciencia, Vol 38, Iss 1, Pp 1-15 (2024)
- Publication Year :
- 2024
- Publisher :
- Universidad Nacional, Costa Rica, 2024.
-
Abstract
- [Objective] This study aimed to evaluate the antimicrobial efficacy of essential oil (EO) from diverse chemotypes of Lippia graveolens against oxytetracycline-resistant Aeromonas hydrophila, which primarily affects the tilapia aquaculture (O. niloticus) in Guatemala. [Methodology] L. graveolens were collected in three departments in Guatemala. The EO was obtained by hydrodistillation and characterized using gas chromatography and mass spectrometry (GC/MS). Subsequently, an antimicrobial assay was conducted using disk and dilution susceptibility tests and evaluation of synergistic interactions among the chemotypes. Each test was performed in triplicate. [Results] The analysis revealed the presence of twenty-seven compounds in the EO obtained from the chemotypes, with the main class being monoterpenes. The major constituents identified were cis-Dihydro-β-terpineol (8.84%) in chemotype I, carvacrol (51.82%) in chemotype II, and thymol (79.62%) in chemotype III. All EO chemotypes of L. graveolens demonstrated the ability to inhibit the A. hydrophila growth. Thymol chemotype exhibited the strongest inhibitory effect against bacterial growth, with a minimum inhibitory concentration (MIC) of 92.4 µg/mL and a minimum bactericidal concentration (MBC) of 184.8 µg/mL. Furthermore, the results suggest that there is no synergistic or additive effect when combining different chemotypes of L. graveolens. [Conclusions] This is the first report of L. graveolens chemotypes exhibiting antimicrobial activity against oxytetracycline-resistant A. hydrophila. The findings suggest that the chemotype thymol could be a potential treatment for infections in the tilapia aquaculture in Guatemala.
- Subjects :
- essential oil
oregano
tilapia
aquaculture
thymol
Science
Science (General)
Q1-390
Subjects
Details
- Language :
- Spanish; Castilian
- ISSN :
- 22153470
- Volume :
- 38
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Uniciencia
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.f8da8282968b4e8a80ccb6103d33d871
- Document Type :
- article
- Full Text :
- https://doi.org/10.15359/ru.38-1.30