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Untargeted Lipidomics after D2O Administration Reveals the Turnover Rate of Individual Lipids in Various Organs of Living Organisms

Authors :
Yury Kostyukevich
Elena Stekolshikova
Anna Levashova
Anna Kovalenko
Anna Vishnevskaya
Anton Bashilov
Albert Kireev
Boris Tupertsev
Lidiia Rumiantseva
Philipp Khaitovich
Sergey Osipenko
Eugene Nikolaev
Source :
International Journal of Molecular Sciences, Vol 24, Iss 14, p 11725 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

The administration of low doses of D2O to living organisms was used for decades for the investigation of metabolic pathways and for the measurement of the turnover rate for specific compounds. Usually, the investigation of the deuterium uptake in lipids is performed by measuring the deuteration level of the palmitic acid residue using GC-MS instruments, and to our knowledge, the application of the modern untargeted LC-MS/MS lipidomics approaches was only reported a few times. Here, we investigated the deuterium uptake for >500 lipids for 13 organs and body liquids of mice (brain, lung, heart, liver, kidney, spleen, plasma, urine, etc.) after 4 days of 100% D2O administration. The maximum deuteration level was observed in the liver, plasma, and lung, while in the brain and heart, the deuteration level was lower. Using MS/MS, we demonstrated the incorporation of deuterium in palmitic and stearic fragments in lipids (PC, PE, TAG, PG, etc.) but not in the corresponding free forms. Our results were analyzed based on the metabolic pathways of lipids.

Details

Language :
English
ISSN :
14220067 and 16616596
Volume :
24
Issue :
14
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.f8931483cbd4dacb3f3b9e52619292c
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms241411725