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Preparation of polymer microspheres capable for pioglitazone release to modify macrophages function
- Source :
- Regenerative Therapy, Vol 11, Iss , Pp 131-138 (2019)
- Publication Year :
- 2019
- Publisher :
- Elsevier, 2019.
-
Abstract
- Introduction: Macrophages play an important role in regulating inflammation and tissue regeneration. It is known that anti-inflammatory macrophages play an important role for tissue regeneration. The objective of this study is to modify macrophages phenotypes for anti-inflammatory function by utilizing drug delivery technology. Method: In this study, 4 types of poly (L-lactic-co-glycolic acid) (PLGA) microspheres incorporating pioglitazone of an anti-inflammatory modifier (pio-MS) with different sizes were prepared. In vitro release test of pio-MS was performed in phosphate buffered-saline solution (PBS) containing 1 wt% of sodium lauryl sulfate. The arginase activity and the secretion of interleukin (IL)−10 as anti-inflammatory macrophage markers of mouse bone marrow derived-macrophages (BMDM) cultured with the pio-MS were evaluated. Results: The sustained release of pioglitazone was observed from all types of pio-MS in vitro. When BMDM were cultured with the pio-MS with an average diameter of 40 μm (pio-MS40), the arginase activity and the secretion of IL-10 increased to a significant extent compared with other pio-MS. Conclusions: The pio-MS40 with an diameter of 40 μm had a potential to induce the anti-inflammatory modification of BMDM in this culture system. The sustained release of pioglitazone is promoting to modify the macrophage function. Keywords: Macrophages, Pioglitazone, Drug delivery system, Poly(L-lactic-co-glycolic acid), PLGA, Microspheres
- Subjects :
- Medicine (General)
R5-920
Cytology
QH573-671
Subjects
Details
- Language :
- English
- ISSN :
- 23523204
- Volume :
- 11
- Issue :
- 131-138
- Database :
- Directory of Open Access Journals
- Journal :
- Regenerative Therapy
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.f852eefc89d94a9d960f2283bb8dd167
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.reth.2019.06.008