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Precise tuning of gene expression levels in mammalian cells

Authors :
Yale S. Michaels
Mike B. Barnkob
Hector Barbosa
Toni A. Baeumler
Mary K. Thompson
Violaine Andre
Huw Colin-York
Marco Fritzsche
Uzi Gileadi
Hilary M. Sheppard
David J. H. F. Knapp
Thomas A. Milne
Vincenzo Cerundolo
Tudor A. Fulga
Source :
Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
Publication Year :
2019
Publisher :
Nature Portfolio, 2019.

Abstract

Analogue regulation of gene expression is important for normal function in mammals but existing genetic technologies are designed to achieve ON/OFF control. Here the authors develop synthetic microRNA silencing-mediated fine-tuners (miSFITs) to precisely control target gene expression levels.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
10
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.f84f595575f647518a682f40b466a3e0
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-019-08777-y