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Detection of micrometastases in peripheral blood of non-small cell lung cancer with a refined immunomagnetic nanoparticle enrichment assay

Authors :
Li Q
Qi H
Zhou HX
Deng CY
Zhu H
Li JF
Wang XL
Li FR
Source :
International Journal of Nanomedicine, Vol 2011, Iss default, Pp 2175-2181 (2011)
Publication Year :
2011
Publisher :
Dove Medical Press, 2011.

Abstract

Qing Li1, Hui Qi1, Han-Xin Zhou1, Chun-Yan Deng1, Hai Zhu3, Jin-Feng Li3, Xi-Li Wang3, Fu-Rong Li1,21Clinical Medical Research Center, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen, People's Republic of China; 2Shenzhen Institute of Gerontology, Shenzhen, People's Republic of China; 3Shenzhen Bioeasy Biotechnologies Co Ltd, Shenzhen, People's Republic of ChinaAbstract: Fe3O4 particles are currently used as the core of immunomagnetic microspheres in the immunomagnetic enrichment assay of circulating tumor cells (CTCs). It is difficult to further improve the sensitivity of CTC detection or to improve tumor cell-type identification and characterization. In the present study, we prepared immunomagnetic nanoparticles with nanopure iron as the core, coated with anti-cytokeratin 7/8 (CK7/8) monoclonal antibody. These immunomagnetic nanoparticles (IMPs) were used in conjunction with immunocytochemistry (ICC) to establish a refined immunomagnetic nanoparticle enrichment assay for CTC detection in non-small cell lung cancer (NSCLC). The assay was compared with nested reverse transcription polymerase chain reaction (RT-PCR) to detect CK19 mRNA and lung specific X protein (LUNX) mRNA. Human lung adenocarcinoma cell line A549 was used for sensitivity and specificity evaluation. Peripheral blood samples were collected from each group for CTC detection. The average diameter of the immunomagnetic nanoparticles was 51 nm, and the amount of adsorbed antibodies was 111.2 µg/mg. We could detect down to one tumor cell in 5 × 107 peripheral blood mononuclear cells. The sensitivity was consistent with that of nested RT-PCR; however, the false positive rate was significantly reduced. The modified assay combined with ICC did not differ from nested RT-PCR in sensitivity, but it had significantly increased specificity. This approach could, therefore, contribute to identification of micrometastases, re-defining clinical staging, and guiding individual postoperative treatments. The technique shows considerable potential clinical value and further clinical trials are warranted.Keywords: NSCLC, circulating tumor cells, nested RT-PCR, immunomagnetic nanoparticles, immunocytochemistry

Subjects

Subjects :
Medicine (General)
R5-920

Details

Language :
English
ISSN :
11769114 and 11782013
Volume :
2011
Issue :
default
Database :
Directory of Open Access Journals
Journal :
International Journal of Nanomedicine
Publication Type :
Academic Journal
Accession number :
edsdoj.f71e88965614b4fa433331e2d25932e
Document Type :
article