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A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain
- Source :
- Synthetic and Systems Biotechnology, Vol 8, Iss 2, Pp 213-219 (2023)
- Publication Year :
- 2023
- Publisher :
- KeAi Communications Co., Ltd., 2023.
-
Abstract
- Nucleic acid detection plays a key role in diverse diagnosis and disease control. Currently available nucleic acid detection techniques are challenged by trade-offs among speed, simplicity, precision and cost. Here, we described a novel method, designated SENSOR (Sulfur DNA mediated nucleic acid sensing platform), for rapid nucleic acid detection. SENSOR was developed from phosphorothioate (PT)-DNA and sulfur binding domain (SBD) which specifically binds double-stranded PT-modified DNA. SENSOR utilizes PT-DNA oligo and SBD as targeting module, which is linked with split luciferase reporter to generate luminescence signal within 10 min. We tested detection on synthesized nucleic acid and COVID-19 pseudovirus, achieving attomolar sensitivity combined with an amplification procedure. Single nucleotide polymorphisms (SNP) could also be discriminated. Indicating SENSOR a new promising nucleic acid detection technique.
Details
- Language :
- English
- ISSN :
- 2405805X
- Volume :
- 8
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- Synthetic and Systems Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.f5af9d909235468f8335028d7813b1d5
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.synbio.2023.02.002