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Polymorphism of Bovine Growth Hormone Receptor Gene (g.3338A>G) and Its Association with Body Measurements and Body Weight in Pasundan Cows

Authors :
W. P. B. Putra
P. P. Agung
S. Anwar
S. Said
Source :
Tropical Animal Science Journal, Vol 42, Iss 2 (2019)
Publication Year :
2019
Publisher :
IPB University, 2019.

Abstract

Bovine growth hormone receptor (bGHR) gene was one of the growth hormone family genes that important for body growth and development of cattle. This research was carried out to identify the polymorphism in the exon 10 of bGHR gene from 142 heads of Pasundan cows at West Java and its association with body length (BL), withers height (WH), heart girth (HG), and body weight (BW). There are two mutation points in the bGHR gene based on the sequencing analysis i.e. g.3322del.A and g.3338A>G. The single nucleotide polymorphism (SNP) at g.3338A>G was detected using PCR-RFLP method with AluI restriction enzyme and showed three genotypes of AA (0.49), AG (0.37), and GG (0.14). The allelic frequencies were 0.67 (A) and 0.33 (G). The number of allele effective (ne) value was 1.79 and described that A allele was the higher allele in the bGHR gene in this study. The polymorphic informative content (PIC) value was 0.34 and categorized as a moderate category. The Chi-square (χ2) analysis showed that the population sample was in the Hardy-Weinberg equilibrium (χ2

Details

Language :
English
ISSN :
2615787X and 2615790X
Volume :
42
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Tropical Animal Science Journal
Publication Type :
Academic Journal
Accession number :
edsdoj.f580d330bb9f4476bed1008d2528201f
Document Type :
article
Full Text :
https://doi.org/10.5398/tasj.2019.42.2.90