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Regulation of CRISPR-Associated Genes by Rv1776c (CasR) in Mycobacterium tuberculosis

Authors :
Wenping Wei
Xiaofang Jiang
Li Zhang
Yunjun Yan
Jinyong Yan
Li Xu
Chun-Hui Gao
Min Yang
Source :
Biomolecules, Vol 13, Iss 2, p 400 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

The CRISPR-Cas system is an adaptive immune system for many bacteria and archaea to defend against foreign nucleic acid invasion, and this system is conserved in the genome of M. tuberculosis (Mtb). Although the CRISPR-Cas system-mediated immune defense mechanism has been revealed in Mtb, the regulation of cas gene expression is poorly understood. In this study, we identified a transcription factor, CasR (CRISPR-associated protein repressor, encoded by Rv1776c), and it could bind to the upstream DNA sequence of the CRISPR-Cas gene cluster and regulate the expression of cas genes. EMSA and ChIP assays confirmed that CasR could interact with the upstream sequence of the csm6 promoter, both in vivo and in vitro. Furthermore, DNA footprinting assay revealed that CasR recognized a 20 bp palindromic sequence motif and negatively regulated the expression of csm6. In conclusion, our research elucidates the regulatory effect of CasR on the expression of CRISPR-associated genes in mycobacteria, thus providing insight into gene expression regulation of the CRISPR-Cas system.

Details

Language :
English
ISSN :
2218273X
Volume :
13
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Biomolecules
Publication Type :
Academic Journal
Accession number :
edsdoj.f56099f3d22b41fdb3180d063bcc2c52
Document Type :
article
Full Text :
https://doi.org/10.3390/biom13020400