Back to Search Start Over

CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil

Authors :
Érica Ribeiro Gomes Lima
Maria Alice Freitas Queiroz
Sandra Souza Lima
Luiz Fernando Almeida Machado
Izaura Maria Vieira Cayres-Vallinoto
Antonio Carlos Rosário Vallinoto
Fernanda Andreza de Pinho Lott Figueiredo
João Farias Guerreiro
Marluísa de Oliveira Guimarães Ishak
Ricardo Ishak
Source :
International Journal of Molecular Sciences, Vol 24, Iss 5, p 4958 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

CCR5Δ32 and SDF1-3′A polymorphisms were investigated in a cohort of viremia controllers, without the use of therapy, along with their influence on CD4+ T lymphocytes (TLs), CD8+ TLs, and plasma viral load (VL). The samples were analyzed from 32 HIV-1-infected individuals classified as viremia controllers 1 and 2 and viremia non-controllers, from both sexes, mostly heterosexuals, paired with 300 individuals from a control group. CCR5∆32 polymorphism was identified by PCR amplification of a fragment of 189 bp for the wild-type allele and 157 bp for the allele with the ∆32 deletion. SDF1-3′A polymorphism was identified by PCR, followed by enzymatic digestion (restriction fragment length polymorphism) with the Msp I enzyme. The relative quantification of gene expression was performed by real-time PCR. The distribution of allele and genotype frequencies did not show significant differences between the groups. The gene expression of CCR5 and SDF1 was not different between the profiles of AIDS progression. There was no significant correlation between the progression markers (CD4+ TL/CD8+ TL and VL) and the CCR5∆32 polymorphism carrier status. The 3′A allele variant was associated with a marked loss of CD4+ TLs and a higher plasma VL. Neither CCR5∆32 nor SDF1-3′A was associated with viremia control or the controlling phenotype.

Details

Language :
English
ISSN :
14220067 and 16616596
Volume :
24
Issue :
5
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.f53a35a9489547dfa0740a224f914889
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms24054958