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Anti-HSV-1 effect of dihydromyricetin from Ampelopsis grossedentata via the TLR9-dependent anti-inflammatory pathway
- Source :
- Journal of Global Antimicrobial Resistance, Vol 23, Iss , Pp 370-376 (2020)
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Objectives: Herpes simplex virus 1 (HSV-1) is one of the most prevalent viruses in humans worldwide. Owing to limited therapeutic options mainly with acyclovir (ACV) and analogues and the emergence of ACV-resistant strains, new drugs with different modes of action and low toxicity are required. The aim of this study was to determine the anti-HSV-1 effect and mechanism of action of the flavonoid compound dihydromyricetin (DHM) from Ampelopsis grossedentata. Methods: The HSV-1 inhibitory effect of DHM was evaluated by measuring plaque formation and generation of progeny virus as well as expression of HSV-1-related genes in Vero cells. The molecular mechanism of the antiviral activity of DHM against HSV-1 was explored by real-time quantitative PCR and ELISA. Results: DHM presented a significant inhibitory effect on HSV-1 plaque formation and generation of progeny virus, with an EC50 (50% effective concentration) of 12.56 μM in Vero cells. Furthermore, expression of HSV-1 immediate-early genes (ICP4 and ICP22), early genes (ICP8 and UL42) and late genes (gB, VP1/2) was decreased by DHM at concentrations of 16 μM and 32 μM. DHM specifically suppressed mRNA levels of Toll-like receptor 9 (TLR9), leading to inhibition of the inflammatory transcriptional factor NFκB and a decrease in TNFα. Conclusion: These findings indicate that the effective inhibitory activity of DHM was achieved by suppressing TNFα production in a TLR9-dependent manner. Although further studies are needed to better characterise the activity of DHM in vivo, the results suggest this extract as a promising new anti-HSV-1 agent.
- Subjects :
- Dihydromyricetin
HSV-1
ICP
Toll-like receptor 9
NF-κB
TNFα
Microbiology
QR1-502
Subjects
Details
- Language :
- English
- ISSN :
- 22137165
- Volume :
- 23
- Issue :
- 370-376
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Global Antimicrobial Resistance
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.f3f7d8c9197a4ce391ba8950df684f66
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.jgar.2020.10.003