Back to Search Start Over

A mimetic peptide of α2,6-sialyllactose promotes neuritogenesis

Authors :
Shuang-Xi Chen
Jia-Hui He
Yong-Jian Mi
Hui-Fan Shen
Melitta Schachner
Wei-Jiang Zhao
Source :
Neural Regeneration Research, Vol 15, Iss 6, Pp 1058-1065 (2020)
Publication Year :
2020
Publisher :
Wolters Kluwer Medknow Publications, 2020.

Abstract

Oxidative stress contributes to the pathogenesis of neurodegenerative diseases. With the aim to find reagents that reduce oxidative stress, a phage display library was screened for peptides mimicking α2,6-sialyllactose (6′-SL), which is known to beneficially influence neural functions. Using Sambucus nigra lectin, which specifically binds to 6′-SL, we screened a phage display library and found a peptide comprising identical sequences of 12 amino acids. Mimetic peptide, reverse peptide and scrambled peptide were tested for inhibition of 6′-SL binding to the lectin. Indeed, lectin binding to 6′-SL was inhibited by the most frequently identified mimetic peptide, but not by the reverse or scrambled peptides, showing that this peptide mimics 6′-SL. Functionally, mimetic peptide, but not the reverse or scrambled peptides, increased viability and expression of neural cell adhesion molecule L1 in SK-N-SH human neuroblastoma cells, and promoted survival and neurite outgrowth of cultured mouse cerebellar granule neurons challenged by H2O2-induced oxidative stress. The combined results indicate that the 6′-SL mimetic peptide promotes neuronal survival and neuritogenesis, thus raising hopes for the treatment of neurodegenerative diseases. This study was approved by the Medical Ethics Committee of Shantou University Medical College, China (approval No. SUMC 2014-004) on February 20, 2014.

Details

Language :
English
ISSN :
16735374
Volume :
15
Issue :
6
Database :
Directory of Open Access Journals
Journal :
Neural Regeneration Research
Publication Type :
Academic Journal
Accession number :
edsdoj.f36ef6bba8464dbdc335f7a6a6adec
Document Type :
article
Full Text :
https://doi.org/10.4103/1673-5374.270313