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Characterization of Green Fluorescent Protein in Heart Valves of a Transgenic Swine Model for Partial Heart Transplant Research

Authors :
Katherine Bishara
Jennie H. Kwon
Morgan A. Hill
Kristi L. Helke
Russell A. Norris
Kristin Whitworth
Randall S. Prather
Taufiek Konrad Rajab
Source :
Journal of Cardiovascular Development and Disease, Vol 10, Iss 6, p 254 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

A transgenic strain of pigs was created to express green fluorescent protein (GFP) ubiquitously using a pCAGG promoter. Here, we characterize GFP expression in the semilunar valves and great arteries of GFP-transgenic (GFP-Tg) pigs. Immunofluorescence was performed to visualize and quantify GFP expression and colocalization with nuclear staining. GFP expression was confirmed in both the semilunar valves and great arteries of GFP-Tg pigs compared to wild-type tissues (aorta, p = 0.0002; pulmonary artery, p = 0.0005; aortic valve; and pulmonic valve, p < 0.0001). The quantification of GFP expression in cardiac tissue allows this strain of GFP-Tg pigs to be used for future research in partial heart transplantation.

Details

Language :
English
ISSN :
23083425
Volume :
10
Issue :
6
Database :
Directory of Open Access Journals
Journal :
Journal of Cardiovascular Development and Disease
Publication Type :
Academic Journal
Accession number :
edsdoj.f34e39bf061447288676e3914e4fc1bc
Document Type :
article
Full Text :
https://doi.org/10.3390/jcdd10060254