Back to Search
Start Over
Isolation and Identification of Ice Nucleation Active Strains from Rapid Apple Declined Trees in Korea
- Source :
- The Plant Pathology Journal, Vol 38, Iss 4, Pp 403-409 (2022)
- Publication Year :
- 2022
- Publisher :
- Hanrimwon Publishing Company, 2022.
-
Abstract
- In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to determine the INA of the strains, using Pseudomonas syringae pv. syringae KACC 21200 as a positive control and resulting in seven INA+ fungal strains that were identified as F. tricinctum (KNUF-21-F17, KNUF-21-F18, KNUF-21-F29, KNUF-21-F32, KNUF-21-F38, KNUF-21-F43, and KNUF-21-F44). The effect of Fusarium INA+ KNUF-21-F29 was compared to that of INA– strains on Chrysanthemum morifolium cv. Shinma explants. A higher callus formation and no-shoot formation were observed, suggesting that fungal INA could play a role in cold injuries and be a factor to consider in rapid apple decline. To the best of our knowledge, this is the first report of INA fungal strains isolated in Korea.
- Subjects :
- cold injury
ice nucleation activity
Plant culture
SB1-1110
Subjects
Details
- Language :
- English
- ISSN :
- 15982254 and 20939280
- Volume :
- 38
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- The Plant Pathology Journal
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.f32c32dfaea343c6be5532804c8b522b
- Document Type :
- article
- Full Text :
- https://doi.org/10.5423/PPJ.NT.04.2022.0051