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Mass spectrometry provides accurate characterization of two genetic marker types in Bacillus anthracis
- Source :
- BioTechniques, Vol 37, Iss 4, Pp 642-651 (2004)
- Publication Year :
- 2004
- Publisher :
- Future Science Ltd, 2004.
-
Abstract
- Epidemiological and forensic analyses of bioterrorism events involving Bacillus anthracis could be improved if both variable number tandem repeats (VNTRs) and single nucleotide polymorphisms (SNPs) could be combined on a single analysis platform. Here we present the use of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) to characterize 24 alleles from 6 VNTR loci and 11 alleles from 7 SNP loci in B. anthracis. The results obtained with ESI-FTICRMS were consistent with independent results obtained from traditional approaches using electrophoretic detection of fluorescent products. However, ESI-FTICR-MS improves on the traditional approaches because it does not require fluorescent labeling of PCR products, minimizes post-PCR processing, obviates electrophoresis, and provides unambiguous base composition of both SNP and VNTR PCR products. In addition, ESI-FTICR-MS allows both marker types to be examined simultaneously and at a rate of approximately 1 sample per min. This technology represents a significant advance in our ability to rapidly characterize B. anthracis isolates using VNTR and SNP loci.
- Subjects :
- Biology (General)
QH301-705.5
Subjects
Details
- Language :
- English
- ISSN :
- 19409818 and 07366205
- Volume :
- 37
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- BioTechniques
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.f2faa25f674507935a47f0e3190f9a
- Document Type :
- article
- Full Text :
- https://doi.org/10.2144/04374RR01