Effect of geographical and agroclimatic location on phytocompounds and antioxidant activity of Moringa oleifera leaves

Moringa oleifera is known has high phytochemical content and biological activity. Differences in geographic and agroclimate can influence the phytochemical content and bioactivity of plants. This study aims to analyze differences in phytochemical content, antioxidant and antibacterial activity of Moringa oleifera leaves obtained from two different environments. Phytochemical content was analyzed qualitatively and quantitatively, bioactivity analysis was carried out by testing antioxidant activity and testing antibacterial activity. Molecular docking predictions for the 3 dominant compounds in both samples as antioxidants was also carried out to Cytochrome c peroxidase. The research results showed that both samples from both locations contained alkaloids, terpenoids, steroids, tannins, saponins, phenols, flavonoids, coumarins, quinones and glycosides. The total phenol and flavonoid content, and antioxidant of sample M1 showed higher than sample M2. From the FTIR analysis, it was confirmed that there were OH, C=O, CO, aliphatic and aromatic carbons functional groups. The most dominant compound in sample M1 based on GC-MS analysis is Levoglucosan (13.6%), while the dominant compound found in sample M2 was 9,12,15-Octadecatrienoic acid, (Z,Z,Z) (16.38%). Molecular docking analysis exhibited that the compound on both sample have promising antioxidant and antibacteria activity. Based on the results, it was revealed that differences in geographical location and agro-climatic conditions influence the phytochemical and bioactivity in Moringa oleifera leaves. These results can be used as a reference in selecting sources of active compounds for pharmacological purposes. [ J Adv Biotechnol Exp Ther 2024; 7(3.000): 556-571]