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Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control Study

Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control Study

Authors :
Maryam Omrizadeh
Pegah Mokhtari
Poopak Eftekhari-Yazdi
Zahra Chekini
Anahita Mohseni Meybodi
Source :
Cell Journal, Vol 24, Iss 9, Pp 540-545 (2022)
Publication Year :
2022
Publisher :
Royan Institute (ACECR), Tehran, 2022.

Abstract

Objective: Diminished ovarian reserve (DOR) is a challenging issue encountered during assisted reproductive technology. Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) belong to the transforming growth factor-beta (TGF-β) superfamily which are essential for folliculogenesis. We aimed to the evaluationof the GDF9 and BMP15 expression in the granulosa cells (GCs) of DOR patients.Materials and Methods: This case-control study included 14 women with DOR and 12 controls, who were between 28-40 years of age undergoing controlled ovarian stimulation with a gonadotropin releasing hormone (GnRH) antagonist protocol. DOR patients were selected by the Bologna criteria. The GCs were extracted from the aspirated follicular fluids and RNA isolated from this. The fold change of gene expressions was assessed by real-time polymerase chain reaction (PCR).Results: GDF9 expression in patients was 0.23 times lower than the control group, which was significant (P

Details

Language :
English
ISSN :
22285806 and 22285814
Volume :
24
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Cell Journal
Publication Type :
Academic Journal
Accession number :
edsdoj.f1ce1dcff6494315811b501c6743af74
Document Type :
article
Full Text :
https://doi.org/10.22074/cellj.2022.8077