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Processing Human Thymic Tissue for Single Cell RNA-Seq
- Source :
- STAR Protocols, Vol 1, Iss 2, Pp 100090- (2020)
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Summary: Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34+ progenitor and more differentiated CD34– fractions from post-natal thymic tissue to study thymopoiesis. CD34+ cells represent
- Subjects :
- Science (General)
Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 26661667
- Volume :
- 1
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- STAR Protocols
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.f1abd48e71fa4d71993df58337025ece
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.xpro.2020.100090