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In-Situ Metabolic Profiling of Different Kinds of Rheum palmatum L. by Laser Desorption–Dielectric Barrier Discharge Ionization Mass Spectrometry Imaging

Authors :
Xue Xiao
Xiaokang Guan
Zhouyi Xu
Qiao Lu
Source :
Metabolites, Vol 14, Iss 3, p 131 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

With its high resolving power and sensitivity, mass spectrometry is considered the most informative technique for metabolite qualitation and quantification in the plant sciences. However, the spatial location information, which is crucial for the exploration of plant physiological mechanisms, is lost. Mass spectrometry imaging (MSI) is able to visualize the spatial distribution of a large number of metabolites from the complex sample surface in a single experiment. In this paper, a flexible and low-cost laser desorption–dielectric barrier discharge ionization-MSI (LD-DBDI-MSI) platform was constructed by combining an LD system with an in-line DBDI source, a high-precision sample translation stage, and an ambient mass spectrometer. It can be operated at a spatial resolution of 20 μm in an atmospheric environment and requires minimal sample preparation. This study presents images of in-situ metabolic profiling of two kinds of plants from different origins, a wild and a farmed Rheum palmatum L. From the screen of these two root sections, the wild one presented five more endogenous molecules than the farmed one, which provides information about the differences in metabolomics.

Details

Language :
English
ISSN :
22181989
Volume :
14
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Metabolites
Publication Type :
Academic Journal
Accession number :
edsdoj.f0dab55a35014ec4b72408823d20d563
Document Type :
article
Full Text :
https://doi.org/10.3390/metabo14030131