P37 Association between inflammation, speed of psychomotor reaction and risk of diabetes

Introduction: The inflammatory JNK (c-Jun N-terminal Kinase) pathway plays a central role in the development of neuroinflammation and type 2 diabetes mellitus (T2DM). The systemic subclinical inflammation is an emblematic feature of T2DM and associated diseases. It is accompanied of activation of inflammatory machrophages which, in a generalized manner, infiltrate and damage a myriad of organs including, among others, CNS and pancreas. Those machrophages, derived from inflammatory monocytes, early overexpress the JNK pathway, even among nondiabetic individuals, especially with obesity. Even though is known that obesity and T2DM impair the CNS, these associations are complex. Prospective studies of high quality have shown that children with high intellectual quotient have a lower prevalence of obesity during adulthood, due to a healthier lifestyle, suggesting that the diabesity-CNS avenue could be bidirectional. Objectives: To explore relationships between the monocyte expression of JNK, the speed of psychomotor reaction (measure correlated with intelligence) and the risk of T2DM. Materials and methods: Design: 102 healthy adults Argentinian women (39.7 ± 13.0 years old), free of any medication, were recruited in a cross-sectional epidemiological study. Measures: the active (phosphorylated) form of JNK (JNK-p) was measured by intracellular ELISA in fresh monocytes obtained by plastic adherence from peripheral blood mononuclear cells purified with the Ficoll/Hypaque method. The speed of psychomotor response to multiple visual stimulus (Four Choice Reaction Time, FCRT) was measured with the test of Deary-Liewald, a software which quantifies in milliseconds their mean (FCRT-M) and standard deviation (FCRT-SD). The risk of T2DM was evaluated with the FINDRISC (FINnish Diabetes Risk SCore) questionnaire, and the insulin resistance with the HOMA-IR.