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Lichenysin-like Polypeptide Production by Bacillus licheniformis B3-15 and Its Antiadhesive and Antibiofilm Properties

Authors :
Vincenzo Zammuto
Maria Giovanna Rizzo
Claudia De Pasquale
Guido Ferlazzo
Maria Teresa Caccamo
Salvatore Magazù
Salvatore Pietro Paolo Guglielmino
Concetta Gugliandolo
Source :
Microorganisms, Vol 11, Iss 7, p 1842 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

We report the ability of the crude biosurfactant (BS B3-15), produced by the marine, thermotolerant Bacillus licheniformis B3-15, to hinder the adhesion and biofilm formation of Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213 to polystyrene and human cells. First, we attempted to increase the BS yield, optimizing the culture conditions, and evaluated the surface-active properties of cell-free supernatants. Under phosphate deprivation (0.06 mM) and 5% saccharose, the yield of BS (1.5 g/L) increased by 37%, which could be explained by the earlier (12 h) increase in lchAA expression compared to the non-optimized condition (48 h). Without exerting any anti-bacterial activity, BS (300 µg/mL) prevented the adhesion of P. aeruginosa and S. aureus to polystyrene (47% and 36%, respectively) and disrupted the preformed biofilms, being more efficient against S. aureus (47%) than P. aeruginosa (26%). When added to human cells, the BS reduced the adhesion of P. aeruginosa and S. aureus (10× and 100,000× CFU/mL, respectively) without altering the epithelial cells’ viability. As it is not cytotoxic, BS B3-15 could be useful to prevent or remove bacterial biofilms in several medical and non-medical applications.

Details

Language :
English
ISSN :
11071842 and 20762607
Volume :
11
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
edsdoj.f0047851506847ce8ff03689a8c093e9
Document Type :
article
Full Text :
https://doi.org/10.3390/microorganisms11071842