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Genome-Wide Association Analysis for Chronic Superficial Keratitis in the Australian Racing Greyhound

Authors :
Steven Karamatic
Rebecca Goode
Niruba Bageswaran
Cali E. Willet
Georgina Samaha
Ray Ferguson
Hamutal Mazrier
Claire M. Wade
Source :
Genes, Vol 13, Iss 8, p 1328 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Chronic superficial keratitis (CSK) is a progressive inflammatory condition of the eye (cornea) that can cause discomfort and blindness. Differential disease risk across dog breeds strongly suggests that CSK has a genetic basis. In addition to genetic risk, the occurrence of CSK is exacerbated by exposure to ultraviolet light. Genome-wide association analysis considered 109 greyhounds, 70 with CSK and the remainder with normal phenotype at an age over four years. Three co-located variants on CFA18 near the 5′ region of the Epidermal Growth Factor Receptor (EGFR) gene were associated with genome-wide significance after multiple-test correction (BICF2P579527, CFA18: 6,068,508, praw = 1.77 × 10−7, pgenome = 0.017; BICF2P1310662, CFA18: 6,077,388, praw = 4.09 × 10−7, pgenome = 0.040; BICF2P160719, CFA18: 6,087,347, praw = 4.09 × 10−7, pgenome = 0.040) (canFam4)). Of the top 10 associated markers, eight were co-located with the significantly associated markers on CFA18. The associated haplotype on CFA18 is protective for the CSK condition. EGFR is known to play a role in corneal healing, where it initiates differentiation and proliferation of epithelial cells that in turn signal the involvement of stromal keratocytes to commence apoptosis. Further validation of the putative functional variants is required prior to their use in genetic testing for breeding programs.

Details

Language :
English
ISSN :
20734425
Volume :
13
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Genes
Publication Type :
Academic Journal
Accession number :
edsdoj.be076787c44189221ec1830dac3f2
Document Type :
article
Full Text :
https://doi.org/10.3390/genes13081328