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Mechanism of human Lig1 regulation by PCNA in Okazaki fragment sealing

Authors :
Kerry Blair
Muhammad Tehseen
Vlad-Stefan Raducanu
Taha Shahid
Claudia Lancey
Fahad Rashid
Ramon Creuhet
Samir M. Hamdan
Alfredo De Biasio
Source :
Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
Publication Year :
2022
Publisher :
Nature Portfolio, 2022.

Abstract

In this work, Blair and co-authors used cryo-EM and in vitro assays to show that human Ligase 1 (Lig1) and Flap Endonuclease 1 (FEN1) form a toolbelt with the sliding clamp PCNA coordinating the sealing of Okazaki fragments.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
13
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.bdb7ac59dbd34e7089e86d0bb7ae4e0a
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-022-35475-z