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Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of Trichinella spiralis in Experimentally Infected Mice

Authors :
Vicente Vega-Sánchez
Fabián-Ricardo Gómez-De-Anda
Georgina Calderón-Domínguez
Mary-Carmen-del-Sol Ramírez-y-Ramírez
Nydia-E. Reyes-Rodríguez
Andrea-P. Zepeda-Velázquez
Raquel Tapia-Romero
Jorge-Luis de-la-Rosa-Arana
Source :
Pathogens, Vol 10, Iss 11, p 1382 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

We study the kinetics of eosinophils during the development of the cellular infiltrate surrounding the nurse cell of Trichinella spiralis (T. spiralis) in experimentally infected mice. Male CD1 mice were experimentally infected with 50 viable muscle larvae of the MSUS/MEX/91/CM-91 T. spiralis strain. Tongues and diaphragms were obtained daily from days 13 to 39 post infection. Diaphragms were compressed and subjected to Giemsa stain. Tongues were histologically sectioned and stained with erythrosine B or hematoxylin and eosin. The cellular infiltrate and the nurse cell-larva complex were detected by optical microscopy since day 16 post infection. The size of the larva increased exponentially during the course of the infection. The kinetics of eosinophils showed a multimodal trend, with a bimodal predominance. The maximum peaks were reached on days 21 and 27 post infection. The results of this study demonstrate that eosinophils occur abundantly in two transcendent moments of the T. spiralis life cycle: first, when the stage 1 larva invades the myocyte and second when the nurse cell-larva complex has been fully developed. These results help one to understand the immunobiology of T. spiralis, highlighting the importance of eosinophils in the survival of the larva in skeletal muscle. Further studies are needed to characterize the cell populations that comprise the cellular infiltrate during the development of the mother cell.

Details

Language :
English
ISSN :
20760817
Volume :
10
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Pathogens
Publication Type :
Academic Journal
Accession number :
edsdoj.bd83b1e07d1645899293585b9959a5b1
Document Type :
article
Full Text :
https://doi.org/10.3390/pathogens10111382