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An automated syringe-based PoC RT-LAMP LFB platform for infectious disease detection from saliva
- Source :
- Frontiers in Lab on a Chip Technologies, Vol 3 (2024)
- Publication Year :
- 2024
- Publisher :
- Frontiers Media S.A., 2024.
-
Abstract
- Decentralized Point-of-Care (PoC) diagnostics hold momentous potential for rapid and accessible viral infection disease detection. Presented is a unique design application of an easy-to-use (plug-and-play) platform for viral detection. The platform leverages a simplified multiplex Reverse-Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Lateral Flow Biosensor (LFB) assay with a lyophilized master mix, eliminating the need for RNA isolation or special reporting equipment. A user-friendly Saliva Measuring Tube (SMT) ensures accurate saliva volume self-collection, and a Syringe-based PoC (SPoC) platform automates sample treatment, reagent mixing, and temperature control using readily available components and consumables. The platform’s performance was evaluated by multiplexed detection of the SARS-CoV-2 N2 target gene and human ACTB gene from saliva samples. The SPoC platform achieved a detection limit of spiked 500 copies/mL for SARS-CoV-2 and consistent internal control readout. The presented PoC system offers a promising initial step for further development toward a decentralized solution for viral infection testing.
Details
- Language :
- English
- ISSN :
- 28133862
- Volume :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Frontiers in Lab on a Chip Technologies
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.bd66cc56917d49d8a55d1a89079a6b3d
- Document Type :
- article
- Full Text :
- https://doi.org/10.3389/frlct.2024.1450891