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An automated syringe-based PoC RT-LAMP LFB platform for infectious disease detection from saliva

Authors :
Dulguunnaran Naranbat
John Murphy
Anubhav Tripathi
Source :
Frontiers in Lab on a Chip Technologies, Vol 3 (2024)
Publication Year :
2024
Publisher :
Frontiers Media S.A., 2024.

Abstract

Decentralized Point-of-Care (PoC) diagnostics hold momentous potential for rapid and accessible viral infection disease detection. Presented is a unique design application of an easy-to-use (plug-and-play) platform for viral detection. The platform leverages a simplified multiplex Reverse-Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Lateral Flow Biosensor (LFB) assay with a lyophilized master mix, eliminating the need for RNA isolation or special reporting equipment. A user-friendly Saliva Measuring Tube (SMT) ensures accurate saliva volume self-collection, and a Syringe-based PoC (SPoC) platform automates sample treatment, reagent mixing, and temperature control using readily available components and consumables. The platform’s performance was evaluated by multiplexed detection of the SARS-CoV-2 N2 target gene and human ACTB gene from saliva samples. The SPoC platform achieved a detection limit of spiked 500 copies/mL for SARS-CoV-2 and consistent internal control readout. The presented PoC system offers a promising initial step for further development toward a decentralized solution for viral infection testing.

Details

Language :
English
ISSN :
28133862
Volume :
3
Database :
Directory of Open Access Journals
Journal :
Frontiers in Lab on a Chip Technologies
Publication Type :
Academic Journal
Accession number :
edsdoj.bd66cc56917d49d8a55d1a89079a6b3d
Document Type :
article
Full Text :
https://doi.org/10.3389/frlct.2024.1450891