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Preparation and characterization of two types of covalently immobilized amyloglucosidase
- Source :
- Journal of the Serbian Chemical Society, Vol 70, Iss 5, Pp 713-719 (2005)
- Publication Year :
- 2005
- Publisher :
- Serbian Chemical Society, 2005.
-
Abstract
- Amyloglucosidase from A. niger was covalently immobilized onto poly (GMA-co-EGDMA) by the glutaraldehyde and periodate method. The immobilization of amyloglucosidase after periodate oxidation gave a preparate with the highest specific activity reported so far on similar polymers. The obtained immobilized preparates show the same pH optimum, but a higher temperature optimum compared with the soluble enzyme. The kinetic parameters for the hydrolysis of soluble starch by free and both immobilized enzymes were determined.
- Subjects :
- glucoamylase
poly(GMA-co-EGDMA)
immobilization
periodate
starch
Chemistry
QD1-999
Subjects
Details
- Language :
- English
- ISSN :
- 03525139
- Volume :
- 70
- Issue :
- 5
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of the Serbian Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.bd1fdd3497db4c6ab2d6b4ea7e796842
- Document Type :
- article