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Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men

Authors :
Anders Berglund
Jaime Matta
Jarline Encarnación-Medina
Carmen Ortiz-Sanchéz
Julie Dutil
Raymond Linares
Joshua Marcial
Caren Abreu-Takemura
Natasha Moreno
Ryan Putney
Ratna Chakrabarti
Hui-Yi Lin
Kosj Yamoah
Carlos Diaz Osterman
Liang Wang
Jasreman Dhillon
Youngchul Kim
Seung Joon Kim
Gilberto Ruiz-Deya
Jong Y. Park
Source :
Biomolecules, Vol 12, Iss 1, p 2 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

In 2021, approximately 248,530 new prostate cancer (PCa) cases are estimated in the United States. Hispanic/Latinos (H/L) are the second largest racial/ethnic group in the US. The objective of this study was to assess DNA methylation patterns between aggressive and indolent PCa along with ancestry proportions in 49 H/L men from Puerto Rico (PR). Prostate tumors were classified as aggressive (n = 17) and indolent (n = 32) based on the Gleason score. Genomic DNA samples were extracted by macro-dissection. DNA methylation patterns were assessed using the Illumina EPIC DNA methylation platform. We used ADMIXTURE to estimate global ancestry proportions. We identified 892 differentially methylated genes in prostate tumor tissues as compared with normal tissues. Based on an epigenetic clock model, we observed that the total number of stem cell divisions (TNSC) and stem cell division rate (SCDR) were significantly higher in tumor than adjacent normal tissues. Regarding PCa aggressiveness, 141 differentially methylated genes were identified. Ancestry proportions of PR men were estimated as African, European, and Indigenous American; these were 24.1%, 64.2%, and 11.7%, respectively. The identification of DNA methylation profiles associated with risk and aggressiveness of PCa in PR H/L men will shed light on potential mechanisms contributing to PCa disparities in PR population.

Details

Language :
English
ISSN :
2218273X
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Biomolecules
Publication Type :
Academic Journal
Accession number :
edsdoj.bbef4b79dbc4d7d960fb890923db350
Document Type :
article
Full Text :
https://doi.org/10.3390/biom12010002