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Ultrasound-Assisted Esterification of Valeric Acid to Alkyl Valerates Promoted by Biosilicified Lipases
- Source :
- Frontiers in Chemistry, Vol 6 (2018)
- Publication Year :
- 2018
- Publisher :
- Frontiers Media S.A., 2018.
-
Abstract
- A novel, environmentally friendly, and sustainable ultrasound-assisted methodology in the valorization of valeric acid to alkyl valerate using a biosilicified lipase from Candida antarctica is reported. This one-pot room temperature methodology of enzyme biosilicification leads to biosilicified lipases with improved activity and reaction efficiency as compared to free enzymes. Yields in the ultrasound-promoted esterification of valeric acid was ca. 90% in 2 h with 15% m/v of biosilicified lipase (Bio-lipase; 616 U/g biocatalyst enzymatic activity) and a molar ratio 1:2 (valeric acid:ethanol), slightly superior to that observed by the free enzyme (75% conversion, 583U/g biocatalyst enzymatic activity). The reuse of enzymes in these conditions was tested and the results show a relatively good reusability of these biosilicified enzymes under the investigated conditions, particularly preserving fairly stable specific activities (616 vs. 430 U/g biocatalyst after four reuses).
- Subjects :
- biosilicification
esterification
ultrasounds
biocatalysis
lipases
Chemistry
QD1-999
Subjects
Details
- Language :
- English
- ISSN :
- 22962646
- Volume :
- 6
- Database :
- Directory of Open Access Journals
- Journal :
- Frontiers in Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.bbb7d5231c5e40fbb4b68f9338214fef
- Document Type :
- article
- Full Text :
- https://doi.org/10.3389/fchem.2018.00197