The Effect of Hydro-alcoholic Extracts of Salvia Officinalis Labiatae and Nasturtium Officinale on Differnt Clinical and Standard Candida Isolates of Candida in Comparison with Fluconazole Drug

Background & aim: Currently, due to the widespread use of antifungal drugs and the inability to treat candida patients with infection, the recurrence of the disease has increased in patients; therefore, this has caused researchers to use medicinal plants to treat fungal diseases. The aim of the present srudy was to investigate the effect of methanolic extract of Nasturtium officinale and Salvia officinalis on the clinical and standard isolates of Candida in comparison with the antifungal drug fluconazole. Methods: In the present experimental study conducted in 2019 on 31 clinical and standard Candida isolates, including 11 Candida albicans isolates, 10 Candida glabrata isolates, and 10 Candida parapsilosis isolates isolated from candidal vaginitis, urinary tract infection, and otomycosis in Yasuj, Iran. Once collecting sage and spring grass, their hydroalcoholic extracts were prepared and used to determine fungal sensitivity by broth microdilution method. For this purpose, according to the standard yeast suspension dilutions along with each of the medicinal agents and plant extracts were added to the 96-well plates and incubated at 35°C. To conclude, the lowest concentration of growth inhibitor was calculated for each isolate. The collected data were analyzed using Excel software. Results: In the present study, among the three drugs and extracts examined, the MIC50 for hydroalcoholic extract of sage on three species of Candida albicans, Candida glabrata and Candida parapsilosis, respectively; 25,000, 12,500 and 12,500 μg/ml and MIC50 for the methanol extract of spring grass on three species in the same way, 25,000 μg/ml and MIC50 for fluconazole for three species; 0.25, 0.5 and 0.125 μg/ml were obtained respectively. Conclusions: Compared to fluconazole, hydroalcoholic extracts of sage and spring grass had much less effect on clinical and standard Candida isolates. The common species that causes candidiasis is Candida albicans, and according to the results of the presnt study, the hydroalcoholic extracts of sage and spring grass have relatively little effects in laboratory conditions; therefore, the use of this plant extract for the treatment of candidiasis is not recommended.