Quality assessment, HPTLC-DPPH, analytical quality by design based HPTLC method development for estimation of piperine in piper species and marketed formulations

Background: Quality assessment of herbs is an essential aspect. In this study HPTLC method is developed by employing the quality based optimization approach with the aid of analytical quality by design tools and the quality assessment of the Piper species. Result: The Quality assessment was carried out for the piper species which was under the acceptance criteria of Ayurvedic Pharmacopeia of India. Eco-friendly and Quality based HPTLC method was developed and validated for Piperine in Extracts as well as marketed formulation. Further the antioxidant potential was checked by the HPTLC-DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Conclusion: Quality assessed extracts and Analytical quality based HPTLC method which was eco-friendly, simple and reliable was developed and validated.