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PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli
- Source :
- Emerging Infectious Diseases, Vol 13, Iss 8, Pp 1253-1253 (2007)
- Publication Year :
- 2007
- Publisher :
- Centers for Disease Control and Prevention, 2007.
-
Abstract
- We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%–20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2.
Details
- Language :
- English
- ISSN :
- 10806040 and 10806059
- Volume :
- 13
- Issue :
- 8
- Database :
- Directory of Open Access Journals
- Journal :
- Emerging Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.b7dc0b42f81947bc96f03b7f57388d90
- Document Type :
- article
- Full Text :
- https://doi.org/10.3201/eid1308.060428