Lactobacillus acidophilus CGMCC 878 impacts colorectal cancer in Sprague-Dawley rats through changing the gut microbiota

A main cause of cancer-related mortality, colorectal cancer (CRC) is one of the most prevalent cancers in the world. A number of variables, including a poor dietary and lifestyle, genetics, metabolic problems, and inheritance, are linked to the risk of CRC. However, it has been studied extensively about the involvement of various environmental factors which facilitates the development of this particular type of cancer in both genders. These elements includes excessive consumption of meat, refined grain and their products, starch, sugars in distinct forms, and alcohol are at top of the list. Hence, to execute the significance of Lactobacillus acidophilus CGMCC 878 (L.A 878) which may possibly hinder the development as well as progression of chemically induce colorectal tumor in in-vivo model via transformation of gut microbiota. We had established CRC in male Sprague-Dawley rats through subcutaneously inject the carcinogen named 1,2 dimethylhydrazine hydrochloride (DMH). We had detected the microbial diversity among different groups which included in the study by evaluation of stool samples by the virtue of molecular genetic computation. Although, we had targeted the bacterial V3 region by using classic molecular technique of PCR-DGGE (Polymerase chain reaction- Denaturing Gradient Gel Electrophoresis) followed by excision of amplified bands and were cloned for sequencing. The DGGE profiles of distinct groups demonstrated significant alterations between DMH group, short-term L. acidophilus group (p ​