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Rehydration of air-dried smears with normal saline: An alternative for conventional wet fixation method in cervical cytological study
- Source :
- Journal of Cytology, Vol 35, Iss 4, Pp 199-203 (2018)
- Publication Year :
- 2018
- Publisher :
- Wolters Kluwer Medknow Publications, 2018.
-
Abstract
- Context: Papanicolaou (Pap) smear is an effective exfoliative cytological investigation done for early recognition of cervical cancer. It also plays role in diagnosis of inflammatory lesions of cervix. Aims: The aim of this study is (1) to compare the cytomorphological features in conventional Pap smear (C-PAPS) and rehydrated air-dried Pap smear (RADPS) and (2) to evaluate the efficacy of RADPS in cytodiagnosis of cervical lesion by comparing with cytomorphological features of conventional wet-fixed Pap smear. Subjects and Methods: Paired cervical smears were prepared for 247 patients. One was labeled as C-PAPS and another was labeled as RADPS. Comparison of both smears was done for various cytomorphological parameters. Results: Out of 247 smears, 2.4% RADPS and 7.3% C-PAPS were reported as unsatisfactory. Red blood cell (RBC) background was present in 2% of RADPS and 42% of C-PAPS. Cytolysis and air-drying artifact were observed more in C-PAPS amounting to 2% and 4% in RADPS and 11% and 15% in C-PAPS. Cytoplasmic staining (97% RADPS vs. 94% C-PAPS) was superior in RADPS. Cell border, nuclear border, and chromatin of squamous and endocervical cells were better appreciated on RADPS compared to C-PAPS, and also statistically significant difference was observed. Conclusion: Rehydrated air-dried technique can be satisfactory alternative for conventional wet fixation method which can be followed routinely or in conjugation with C-PAPS, especially in cervical screening programs.
Details
- Language :
- English
- ISSN :
- 09709371
- Volume :
- 35
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Cytology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.b703b8276c29481ab3be5c4ba26c5326
- Document Type :
- article
- Full Text :
- https://doi.org/10.4103/JOC.JOC_186_17