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Snake venoms and purified toxins as biotechnological tools to control Ralstonia solanacearum

Authors :
Rita de Cássia Alves
José Roberto Vieira Júnior
Tamiris Chaves Freire
Aline Souza da Fonseca
Simone Carvalho Sangi
Fábio da Silva Barbieri
Rodrigo Barros Rocha
Luciana Gatto Brito
Soraya dos Santos Pereira
Marcos Barros Luiz
Francisco das Chagas Oliveira Freire
Carla Freire Celedonio Fernandes
Andreimar Martins Soares
Cléberson de Freitas Fernandes
Source :
Pesquisa Agropecuária Brasileira, Vol 55 (2020)
Publication Year :
2020
Publisher :
Embrapa Informação Tecnológica, 2020.

Abstract

Abstract: The objective of this work was to evaluate the in vitro antibacterial activity of snake venoms and purified toxins on the phytopathogenic bacterium Ralstonia solanacearum. The evaluations were performed with 17 crude venoms (13 from Bothrops, 3 from Crotalus, and 1 from Lachesis) and seven toxins (1 from Bothrops and 6 from Crotalus). Antibacterial activity was assessed in MB1 medium containing solubilized treatments (1 μL mL-1). A total of 100 μL bacterial suspension (8.4 x 109 CFU mL-1) was used. After incubation at 28°C, the number of bacterial colonies at 24, 48, and 72 hours after inoculation was evaluated. SDS-PAGE gel at 15% was used to analyze the protein patterns of the samples, using 5 μg protein of each sample in the assay. Furthermore, the minimum inhibitory concentration (MIC) and lethal concentration (LC50) values were determined by the Probit method. Venoms and toxins were able to reduce more than 90% of R. solanacearum growth. These results were either equivalent to those of the positive control chloramphenicol or even better. While MIC values ranged from 4.0 to 271.5 μg mL-1, LC50 ranged from 28.5 μg mL-1 to 4.38 mg mL-1. Ten crude venoms (7 from Bothrops and 3 from Crotalus) and two purified toxins (gyroxin and crotamine) are promising approaches to control the phytopathogenic bacterium R. solanacearum.

Details

Language :
English, Spanish; Castilian, Portuguese
ISSN :
16783921
Volume :
55
Database :
Directory of Open Access Journals
Journal :
Pesquisa Agropecuária Brasileira
Publication Type :
Academic Journal
Accession number :
edsdoj.b6130549735f44e5a0fa95f396dfa50c
Document Type :
article
Full Text :
https://doi.org/10.1590/s1678-3921.pab2020.v55.01756