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Chemical Protein Crosslinking-Coupled Mass Spectrometry Reveals Interaction of LHCI with LHCII and LHCSR3 in Chlamydomonas reinhardtii

Authors :
Laura Mosebach
Shin-Ichiro Ozawa
Muhammad Younas
Huidan Xue
Martin Scholz
Yuichiro Takahashi
Michael Hippler
Source :
Plants, Vol 13, Iss 12, p 1632 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

The photosystem I (PSI) of the green alga Chlamydomonas reinhardtii associates with 10 light-harvesting proteins (LHCIs) to form the PSI-LHCI complex. In the context of state transitions, two LHCII trimers bind to the PSAL, PSAH and PSAO side of PSI to produce the PSI-LHCI-LHCII complex. In this work, we took advantage of chemical crosslinking of proteins in conjunction with mass spectrometry to identify protein–protein interactions between the light-harvesting proteins of PSI and PSII. We detected crosslinks suggesting the binding of LHCBM proteins to the LHCA1-PSAG side of PSI as well as protein–protein interactions of LHCSR3 with LHCA5 and LHCA3. Our data indicate that the binding of LHCII to PSI is more versatile than anticipated and imply that LHCSR3 might be involved in the regulation of excitation energy transfer to the PSI core via LHCA5/LHCA3.

Details

Language :
English
ISSN :
22237747
Volume :
13
Issue :
12
Database :
Directory of Open Access Journals
Journal :
Plants
Publication Type :
Academic Journal
Accession number :
edsdoj.b59fd2a6d9d9444a93f113df981fb822
Document Type :
article
Full Text :
https://doi.org/10.3390/plants13121632