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DNAzyme-Amplified Electrochemical Biosensor Coupled with pH Meter for Ca2+ Determination at Variable pH Environments

Authors :
Hui Wang
Fan Zhang
Yue Wang
Fangquan Shi
Qingyao Luo
Shanshan Zheng
Junhong Chen
Dingzhen Dai
Liang Yang
Xiangfang Tang
Benhai Xiong
Source :
Nanomaterials, Vol 12, Iss 1, p 4 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

For more than 50% of multiparous cows, it is difficult to adapt to the sudden increase in calcium demand for milk production, which is highly likely to cause hypocalcemia. An electrochemical biosensor is a portable and efficient method to sense Ca2+ concentrations, but biomaterial is easily affected by the pH of the analyte solution. Here, an electrochemical biosensor was fabricated using a glassy carbon electrode (GCE) and single-walled carbon nanotube (SWNT), which amplified the impedance signal by changing the structure and length of the DNAzyme. Aiming at the interference of the pH, the electrochemical biosensor (GCE/SWNT/DNAzyme) was coupled with a pH meter to form an electrochemical device. It was used to collect data at different Ca2+ concentrations and pH values, and then was processed using different mathematical models, of which GPR showed higher detecting accuracy. After optimizing the detecting parameters, the electrochemical device could determine the Ca2+ concentration ranging from 5 μM to 25 mM, with a detection limit of 4.2 μM at pH values ranging from 4.0 to 7.5. Finally, the electrochemical device was used to determine the Ca2+ concentrations in different blood and milk samples, which can overcome the influence of the pH.

Details

Language :
English
ISSN :
20794991
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nanomaterials
Publication Type :
Academic Journal
Accession number :
edsdoj.b55904e3d1774eb6ba931b69fe63c746
Document Type :
article
Full Text :
https://doi.org/10.3390/nano12010004