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PRC1 Prevents Replication Stress during Chondrogenic Transit Amplification

Authors :
Frank Spaapen
Lars M. T. Eijssen
Michiel E. Adriaens
Tim J. Welting
Peggy Prickaerts
Juliette Salvaing
Vivian E. H. Dahlmans
Donald A. M. Surtel
Frans Kruitz
Roel Kuijer
Yoshihiro Takihara
Hendrik Marks
Hendrik G. Stunnenberg
Bradly G. Wouters
Miguel Vidal
Jan Willem Voncken
Source :
Epigenomes, Vol 1, Iss 3, p 22 (2017)
Publication Year :
2017
Publisher :
MDPI AG, 2017.

Abstract

Transit amplification (TA), a state of combined, rapid proliferative expansion and differentiation of stem cell-descendants, remains poorly defined at the molecular level. The Polycomb Repressive Complex 1 (PRC1) protein BMI1 has been localized to TA compartments, yet its exact role in TA is unclear. PRC1 proteins control gene expression, cell proliferation and DNA-damage repair. Coordination of such DNA-templated activities during TA is predicted to be crucial to support DNA replication and differentiation-associated transcriptional programming. We here examined whether chondrogenesis provides a relevant biological context for synchronized coordination of these chromatin-based tasks by BMI1. Taking advantage of a prominently featuring TA-phase during chondrogenesis in vitro and in vivo, we here report that TA is completely dependent on intact PRC1 function. BMI1-depleted chondrogenic progenitors rapidly accumulate double strand DNA breaks during DNA replication, present massive non-H3K27me3-directed transcriptional deregulation and fail to undergo chondrogenic TA. Genome-wide accumulation of Topoisomerase 2α and Geminin suggests a model in which PRC1 synchronizes replication and transcription during rapid chondrogenic progenitor expansion. Our combined data reveals for the first time a vital cell-autonomous role for PRC1 during chondrogenesis. We provide evidence that chondrocyte hyper-replication and hypertrophy represent a unique example of programmed senescence in vivo. These findings provide new perspectives on PRC1 function in development and disease.

Details

Language :
English
ISSN :
20754655
Volume :
1
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Epigenomes
Publication Type :
Academic Journal
Accession number :
edsdoj.b4b3f61c4d34a93b35991f74ff5772b
Document Type :
article
Full Text :
https://doi.org/10.3390/epigenomes1030022