Enhancing signal-to-noise ratio in real-time LED-based photoacoustic imaging: A comparative study of CNN-based deep learning architectures

Recent advances in Light Emitting Diode (LED) technology have enabled a more affordable high frame rate photoacoustic imaging (PA) alternative to traditional laser-based PA systems that are costly and have slow pulse repetition rate. However, a major disadvantage with LEDs is the low energy outputs that do not produce high signal-to-noise ratio (SNR) PA images. There have been recent advancements in integrating deep learning methodologies aimed to address the challenge of improving SNR in LED-PA images, yet comprehensive evaluations across varied datasets and architectures are lacking. In this study, we systematically assess the efficacy of various Encoder-Decoder-based CNN architectures for enhancing SNR in real-time LED-based PA imaging. Through experimentation with in vitro phantoms, ex vivo mouse organs, and in vivo tumors, we compare basic convolutional autoencoder and U-Net architectures, explore hierarchical depth variations within U-Net, and evaluate advanced variants of U-Net. Our findings reveal that while U-Net architectures generally exhibit comparable performance, the Dense U-Net model shows promise in denoising different noise distributions in the PA image. Notably, hierarchical depth variations did not significantly impact performance, emphasizing the efficacy of the standard U-Net architecture for practical applications. Moreover, the study underscores the importance of evaluating robustness to diverse noise distributions, with Dense U-Net and R2 U-Net demonstrating resilience to Gaussian, salt and pepper, Poisson, and Speckle noise types. These insights inform the selection of appropriate deep learning architectures based on application requirements and resource constraints, contributing to advancements in PA imaging technology.