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Label-free approaches for extracellular vesicle detection

Authors :
Loredana Leggio
Greta PaternĂ²
Silvia Vivarelli
Aurelio Bonasera
Bruno Pignataro
Nunzio Iraci
Giuseppe Arrabito
Source :
iScience, Vol 26, Iss 11, Pp 108105- (2023)
Publication Year :
2023
Publisher :
Elsevier, 2023.

Abstract

Summary: Extracellular vesicles (EVs) represent pivotal mediators in cell-to-cell communication. They are lipid-membranous carriers of several biomolecules, which can be produced by almost all cells. In the current Era of precision medicine, EVs gained growing attention thanks to their potential in both biomarker discovery and nanotherapeutics applications. However, current technical limitations in isolating and/or detecting EVs restrain their standard use in clinics. This review explores all the state-of-the-art analytical technologies which are currently overcoming these issues. On one end, several innovative optical-, electrical-, and spectroscopy-based detection methods represent advantageous label-free methodologies for faster EV detection. On the other end, microfluidics-based lab-on-a-chip tools support EV purification from low-concentrated samples. Altogether, these technologies will strengthen the routine application of EVs in clinics.

Details

Language :
English
ISSN :
25890042
Volume :
26
Issue :
11
Database :
Directory of Open Access Journals
Journal :
iScience
Publication Type :
Academic Journal
Accession number :
edsdoj.b207794b2724f29b470298fcd63f320
Document Type :
article
Full Text :
https://doi.org/10.1016/j.isci.2023.108105