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Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.

Authors :
Hee Jae Shin
Byeoung-Kyu Choi
Phan Thi Hoai Trinh
Hwa-Sun Lee
Jong Soon Kang
Tran Thi Thanh Van
Hyi-Seung Lee
Jong Seok Lee
Yeon-Ju Lee
Jihoon Lee
Source :
Marine Drugs, Vol 16, Iss 1, p 14 (2018)
Publication Year :
2018
Publisher :
MDPI AG, 2018.

Abstract

A new α-pyrone merosesquiterpenoid possessing an angular tetracyclic carbon skeleton, ochraceopone F (1), and four known secondary metabolites, aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), and mactanamide (5), were isolated from the marine fungus Aspergillus flocculosus derived from a sponge Stylissa sp. collected in Vietnam. The structures of Compounds 1–5 were elucidated by analysis of 1D and 2D NMR spectra and MS data. All the isolated compounds were evaluated for anti-proliferation activity and their suppression effects on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclast differentiation using tartate-resisant acid phosphatase (TRAP). Compounds 1–5 had no anti-proliferative effect on human cancer cell lines up to 30 μg/mL. Among these compounds, aspertetranone D (2) and wasabidienone E (4) exhibited weak osteoclast differentiation inhibitory activity at 10 μg/mL. However, mactanamide (5) showed a potent suppression effect of osteoclast differentiation without any evidence of cytotoxicity.

Details

Language :
English
ISSN :
16603397
Volume :
16
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Marine Drugs
Publication Type :
Academic Journal
Accession number :
edsdoj.b1d7ce26093845cd8e6bbc89fa10b86e
Document Type :
article
Full Text :
https://doi.org/10.3390/md16010014