A Theoretical Mechanism for the Action of SONG-Modulated Laser Light on Human Very Small Embryonic-Like (hVSEL) Stem Cells in Platelet Rich Plasma (PRP)

It has previously been shown that human very small embryonic-like (hVSEL) stem cell proliferation occurs rapidly when hVSEL stem cells in platelet rich plasma (PRP) are exposed to Strachan Ovokaitys Node Generator (SONG)-modulated laser light. The surface antigens Oct 3/4, SSEA4 and CXCR4 in the lineage negative (Lin-) compartment were assessed using flow cytometry. Of these three markers, it is known that CXCR4 may be blocked from binding to flow cytometry antibodies by its antagonist ligand, the Endogenous Peptide Inhibitor of CXCR4 (EPI-X4). In this theoretical manuscript, we focus on possible novel methods of unblocking CXCR4 by SONG-modulated laser light to make it readily available for binding by flow cytometry antibodies. We propose that the SONG-modulated red laser penetrates the minor pocket of CXCR4 and thus disrupts the hydrogen bonds and salt bridges binding CXCR4 to EPI-X4. A quantum-mechanical description of the laser interaction with the hydrogen atoms of the hydrogen bonds and salt bridges provides an atom-level illustration of the forces producing stem cell proliferation effects in vitro. This is a novel description of the mechanism of action of SONG-modulated laser light on stem cell antigens at the quantum level which may have wide implications in stem cell biology and regenerative medicine.