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MiR-548ar-3p increases cigarette smoke extractinduced chronic obstructive pulmonary disease (COPD) injury through solute carrier family 17 member 9 (SLC17A9)
- Source :
- Archives of Biological Sciences, Vol 74, Iss 1, Pp 97-105 (2022)
- Publication Year :
- 2022
- Publisher :
- University of Belgrade, University of Novi Sad, 2022.
-
Abstract
- This study investigated the effect of microRNA mir-548ar-3p on cigarette smoke extract (CSE)-induced chronic obstructive pulmonary disease (COPD). High-throughput sequencing was performed on peripheral blood from smoking COPD patients and non-smoking individuals with normal pulmonary function, and miR-548ar-3p RNA, possessing large differential expression was selected. Experimental groups were divided into control, experimental model (EM), EM+mimic miRNA, negative control (NC) and EM+miR-548ar-3p groups; an empty vector or miR-548ar-3p mimic was transfected into human bronchial epithelial (HBE) cells. A COPD model was established by treating HBE cells with CSE. Cell viability, apoptosis and solute carrier family 17 member 9 (SLC17A9) protein expression were examined by cell counting kit-8, flow cytometry and Western blotting, respectively. Cell viability in the EM+miR-548ar-3p group decreased significantly, and the apoptosis rate and SLC17A9 protein expression increased significantly compared with the control (P
Details
- Language :
- English
- ISSN :
- 03544664 and 18214339
- Volume :
- 74
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Archives of Biological Sciences
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.b0e80d3536784b209e6bd9a84b9f3ecc
- Document Type :
- article
- Full Text :
- https://doi.org/10.2298/ABS220201008Z