Magnesium: does it reduce ischemia/reperfusion injury in an adnexal torsion rat model?

Ebru Celik Kavak,1 Funda Gulcu Bulmus,2 Ozgur Bulmus,3 Salih Burcin Kavak,1 Nevin Kocaman4 1Department of Obstetrics and Gynecology, 2Vocational School of Health Services, 3Department of Physiology, 4Department of Histology and Embryology, School of Medicine, Firat University, Elazig, Turkey Aim: The aim of the present study was to assess the protective effects of magnesium sulfate (MgSO4) on ischemia/reperfusion (I/R) induced ovarian damage in a rat ovarian torsion model.Methods: Forty-two female Sprague Dawley rats were included in the study. They were divided into six groups as Group 1, sham; Group 2, bilateral ovarian torsion; Group 3, bilateral ovarian torsion–detorsion; Group 4, MgSO4–sham; Group 5, MgSO4–bilateral ovarian torsion; Group 6, bilateral ovarian torsion–MgSO4–detorsion. Both torsion and detorsion periods lasted 3 hours. In Groups 4, 5 and 6, MgSO4 (600 mg/kg) was administered by intraperitoneal route 30 minutes before sham operation, torsion and detorsion, respectively. At the end of the study period, both ovaries were removed. One of the ovaries was used for histopathological analyses and the other for biochemical analyses.Results: In the torsion–detorsion group, all the histopathological scores were higher compared to the sham and torsion only group (p